BMP4 Chemi-Luminescent ELISA Kit (Human) (OKCD03332)

Catalog No: OKCD03332

Size:96WELLS

Human BMP4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-101%	89-98%	80-97%	79-94%
  EDTA Plasma (n=5)	94-102%	82-89%	88-104%	91-101%
  Heparin Plasma (n=5)	89-99%	98-105%	86-104%	80-90%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-90	86
  EDTA Plasma (n=5)	80-105	102
  Heparin Plasma (n=5)	78-90	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BMP4 Microplate: 96 Wells (12 x 8 Well strips)
  • BMP4 Lyophilized Standard: 2
  • 100X BMP4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 2.74 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BMP4
• Gene Full Name: bone morphogenetic protein 4
• Alias Symbols: BMP2B;BMP2B1;bone morphogenetic protein 2B;bone morphogenetic protein 4;MCOPS6;OFC11;ZYME.
• NCBI Gene Id: 652
• Protein Name: Bone morphogenetic protein 4
• Description of Target: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
• Uniprot ID: P12644
• Protein Accession #: NP_001193
• Nucleotide Accession #: NM_001202