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Catalog No: OKCD00737 (Formerly GWB-KBBWI9)
Size:96WELLS
Price: $725.00
SKU
OKCD00737
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Datasheets/ManualsPrintable datasheet for BMP2 ELISA Kit (Pig) (OKCD00737)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-98%90-103%96-103%90-105%
EDTA plasma(n=5)89-97%79-90%83-97%88-97%
heparin plasma(n=5)80-90%84-93%96-105%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 2 (BMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 2 (BMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.25-2000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)91-10496
EDTA plasma(n=5)93-10196
heparin plasma(n=5)91-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BMP2 Microplate96 Wells (12 x 8 Well Strips)
BMP2 Lyophilized Standard2 x 2 ng
100X Biotinylated BMP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 13.1 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2). No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBMP2
Gene Full Namebone morphogenetic protein 2
Alias Symbolsbone morphogenetic protein 2.
NCBI Gene Id100157103
Protein NameBone morphogenetic protein 2
Description of TargetBone morphogenetic protein 2
Uniprot IDE1AXT2
Protein Accession #XP_001928064  
Nucleotide Accession #XM_001928029.4
Protein Size (# AA)395
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