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Catalog No: OKEH00026
Size:96T
Price: $325.00
SKU
OKEH00026
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00026
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BMP2 ELISA Kit (Mouse) (OKEH00026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMP2 captured in well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
BMP2 Microplate96 Wells (12 x 8 Well strips)
BMP2 Lyophilized Standard2
100X Biotinylated BMP2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity31.25 pg/mL
SpecificityNatural and recombinant Mouse Bone morphogenetic protein 2
Publications

Noggin Inhibits IL-1β and BMP-2 Expression, and Attenuates Cartilage Degeneration and Subchondral Bone Destruction in Experimental Osteoarthritis. Cells. 9, (2020). 32290085

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolBmp2
Gene Full Namebone morphogenetic protein 2
Alias SymbolsAI467020;Bmp;Bmp2a;bone morphogenetic protein 2;bone morphogenetic protein 2A.
NCBI Gene Id12156
Protein NameP21274
Description of TargetBone morphogenetic protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/P21274
Protein Accession #NP_031579
Nucleotide Accession #NM_007553
Protein Size (# AA)394
Molecular Weight45 kDa
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