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Catalog No: OKCD03326
Size:96WELLS
Price: $850.00
SKU
OKCD03326
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BMP2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03326)

Datasheets/ManualsPrintable datasheet for BMP2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03326)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)83-97%85-95%96-103%80-94%
EDTA Plasma (n=5)94-105%83-104%93-101%83-97%
Heparin Plasma (n=5)99-105%91-99%97-104%86-98%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 2 (BMP2) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)93-10397
EDTA Plasma (n=5)88-9991
Heparin Plasma (n=5)85-9995
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Bmp2 Microplate96 Wells (12 x 8 Well strips)
Bmp2 Lyophilized Standard2
100X Bmp2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBmp2
Gene Full Namebone morphogenetic protein 2
Alias SymbolsAI467020;Bmp;Bmp2a;bone morphogenetic protein 2;bone morphogenetic protein 2A.
NCBI Gene Id12156
Protein NameBone morphogenetic protein 2
Description of TargetInduces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Uniprot IDP21274
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