Catalog No: OKCD02401
Size:96 Wells
Price: $625.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration3 Hours
ELISA Kit Linearity
Serum (n=5)96-103%98-105%95-104%91-102%
EDTA Plasma (n=5)96-104%83-98%96-103%79-105%
Heparin Plasma (n=5)80-102%82-94%97-104%98-105%
ELISA Kit PrincipleAviva Systems Biology Bmp1 ELISA Kit (Mouse) (OKCD02401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Bmp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Bmp1 captured in well.
ELISA Kit Range78.12-5,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-9592
EDTA Plasma (n=5)92-10196
Heparin Plasma(n=5)78-9484
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level BMP1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level BMP1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
ELISA Kit Component
Anti-BMP1 Microplate96 Wells (12 x 8 Well strips)
BMP1 Lyophilized Standard2
100X Biotinylated BMP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity32 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 1 (BMP1). No significant cross-reactivity or interference between Bone Morphogenetic Protein 1 (BMP1) and analogues was observed.

Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolBMP1
Gene Full Namebone morphogenetic protein 1
Alias SymbolsPcp, Tld
NCBI Gene Id12153
Protein NameBone morphogenetic protein 1
Description of TargetCleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX.
Uniprot IDP98063
Protein Accession #NP_033885.2
Nucleotide Accession #NM_009755.3
Protein Size (# AA)991
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