BMP1 ELISA Kit (Mouse) (OKCD02401)

Catalog No: OKCD02401

Size:96 Wells

Mouse BMP1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 3 Hours
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-103%	98-105%	95-104%	91-102%
  EDTA Plasma (n=5)	96-104%	83-98%	96-103%	79-105%
  Heparin Plasma (n=5)	80-102%	82-94%	97-104%	98-105%

• ELISA Kit Principle: Aviva Systems Biology Bmp1 ELISA Kit (Mouse) (OKCD02401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Bmp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Bmp1 captured in well.
• ELISA Kit Range: 78.12-5,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-95	92
  EDTA Plasma (n=5)	92-101	96
  Heparin Plasma(n=5)	78-94	84

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level BMP1 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level BMP1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10% (n=20)
  Inter-Assay CV: <12% (n=24)
• ELISA Kit Component:
  • Anti-BMP1 Microplate: 96 Wells (12 x 8 Well strips)
  • BMP1 Lyophilized Standard: 2
  • 100X Biotinylated BMP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 1 (BMP1). No significant cross-reactivity or interference between Bone Morphogenetic Protein 1 (BMP1) and analogues was observed.
• Publications:

  Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BMP1
• Gene Full Name: bone morphogenetic protein 1
• Alias Symbols: Pcp, Tld
• NCBI Gene Id: 12153
• Protein Name: Bone morphogenetic protein 1
• Description of Target: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX.
• Uniprot ID: P98063
• Protein Accession #: NP_033885.2
• Nucleotide Accession #: NM_009755.3
• Protein Size (# AA): 991