Catalog No: OKCD02401
Price: $625.00
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Datasheets/ManualsPrintable datasheet for BMP1 ELISA Kit (Mouse) (OKCD02401)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Serum (n=5)96-103%98-105%95-104%91-102%
EDTA Plasma (n=5)96-104%83-98%96-103%79-105%
Heparin Plasma (n=5)80-102%82-94%97-104%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 1 (BMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 1 (BMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 1 (BMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 1 (BMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-9592
EDTA Plasma (n=5)92-10196
Heparin Plasma(n=5)78-9484
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-BMP1 Microplate96 Wells (12 x 8 Well strips)
BMP1 Lyophilized Standard2
100X Biotinylated BMP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity32 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 1 (BMP1). No significant cross-reactivity or interference between Bone Morphogenetic Protein 1 (BMP1) and analogues was observed.

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Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolBmp1
Gene Full Namebone morphogenetic protein 1
Alias Symbolsbone morphogenetic protein 1;mammalian tolloid protein;Pcp;procollagen C-proteinase;Tld.
NCBI Gene Id12153
Protein NameBone morphogenetic protein 1
Description of TargetCleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD) (By similarity). Responsible for the proteolytic activation of lysyl oxidase LOX.
Uniprot IDP98063
Protein Accession #NP_033885.2
Nucleotide Accession #NM_009755.3
Protein Size (# AA)991
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