Bmp1 Antibody - C-terminal region (OAAB12551)

Catalog No: OAAB12551

Size:400ul

Bmp1 Antibody (R972) (Cat. # OAAB12551) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Bmp1 antibody detected the Bmp1 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC-P, WB
  Function: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Responsible for the proteolytic activation of lysyl oxidase LOX.
  Cellular Location: Golgi apparatus, trans-Golgi network. Secreted, extracellular space, extracellular matrix. Note=Co- localizes with POSTN in the Golgi.
  Tissue Location: Ubiquitous.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Bmp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 957-986 amino acids from the C-terminal region of human Bmp1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: IHC-P: 1:50~100
  WB: 1:1000

Target Info

• Gene Symbol: BMP1
• Alias Symbols: PCP, TLD, OI13, PCP2, PCOLC
• NCBI Gene Id: 649
• Protein Name: Bone morphogenetic protein 1
• Description of Target: The BMP1 locus encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, BMP1 encodes a protein that is not closely related to other known growth factors. BMP1 protein and procollagen C proteinase (PCP), a secreted metalloprotease requiring calcium and needed for cartilage and bone formation, are identical. PCP or BMP1 protein cleaves the C-terminal propeptides of procollagen I, II, and III and its activity is increased by the procollagen C-endopeptidase enhancer protein. The BMP1 gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region.
• Uniprot ID: P13497
• Protein Accession #: NP_001190.1
• Molecular Weight: 111 kDa