BMP-2 ELISA Kit (Rat) (OKBB00132)

Catalog No: OKBB00132

Size:96 Tests

Rat BMP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat BMP-2 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	349	1058	2437	357	1068	2479
  St. Dev.	10.1	33.9	82.9	23.2	75.8	178.5
  %CV	2.89	3.20	3.40	6.49	7.09	7.20

• ELISA Kit Component:
  • Lyophilized recombinant rat BMP-2 standard: 10 ng/tube x 2
  • Anti-rat BMP-2 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-rat BMP-2 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 62.5pg/ml-4000pg/ml
  Principle: Aviva’s rat BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat BMP-2 specific-specific monoclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMP-2 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: bone tissue, cell culture supernates and serum
• Sensitivity: <2 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine.
• Reference: 1. Gopal Rao, V. V. N.; Loffler, C.; Wozney, J. M.; Hansmann, I. The gene for bone morphogenetic protein 2A
  (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum.
  Genet. 90: 299-302, 1992.
  2. Hallahan, A. R.; Pritchard, J. I.; Chandraratna, R. A. S.; Ellenbogen, R. G.; Geyer, J. R.; Overland, R. P.; Strand, A.
  D.; Tapscott, S. J.; Olson, J. M. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a
  paracrine effect. Nature Med. 9: 1033-1038, 2003.
  3. Cheng, H.; Jiang, W.; Phillips, F. M.; Haydon, R. C.; Peng, Y.; Zhou, L.; Luu, H. H.; An, N.; Breyer, B.; Vanichakarn,
  P.; Szatkowski, J. P.; Park, J. Y.; He, T.-C. Osteogenic activity of the fourteen types of human bone morphogenetic
  proteins (BMPs). J. Bone Joint Surg. 85-A: 1544-1552, 2003.
• Specificity: Natural and recombinant rat BMP-2
• Immunogen: Expression system for standard: CHO; Immunogen sequence: Q283-R396
• Description: For quantitative detection of rat BMP-2 in bone tissue, cell culture supernatants and serum.

Target Info

• Gene Symbol: BMP2
• Gene Full Name: bone morphogenetic protein 2
• Alias Symbols: BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A
• NCBI Gene Id: 29373
• Protein Name: Bone morphogenetic protein 2
• Description of Target: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
• Uniprot ID: P49001
• Protein Accession #: NP_058874.1
• Nucleotide Accession #: NM_017178.1