Catalog No: OKCD00982 (Formerly GWB-KBBZB2)
Size:96WELLS
Price: $675.00
SKU
OKCD00982
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Datasheets/ManualsPrintable datasheet for BMI1 ELISA Kit (Human) (OKCD00982)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polycomb Group Ring Finger Protein 4 (PCGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Polycomb Group Ring Finger Protein 4 (PCGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BMI1 Microplate96 Wells (12 x 8 Well Strips)
BMI1 Lyophilized Standard2 x 20 ng
100X Biotinylated BMI1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.122 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Polycomb Group Ring Finger Protein 4 (PCGF4). No significant cross-reactivity or interference between Polycomb Group Ring Finger Protein 4 (PCGF4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBMI1|COMMD3-BMI1
Gene Full NameBMI1 proto-oncogene, polycomb ring finger|COMMD3-BMI1 readthrough
Alias SymbolsB lymphoma Mo-MLV insertion region 1 homolog;BMI1;BMI1 polycomb ring finger oncogene;BMI1 polycomb ring finger proto-oncogene;COMMD3-BMI1 read-through protein;flvi-2/bmi-1;FLVI2/BMI1;murine leukemia viral (bmi-1) oncogene homolog;PCGF4;polycomb complex protein BMI-1;polycomb group protein Bmi1;polycomb group RING finger protein 4;ring finger protein 51;RNF51.
NCBI Gene Id100532731|648
Protein NamePolycomb complex protein BMI-1
Description of TargetComponent of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
Uniprot IDP35226
Protein Accession #NP_001190991
Nucleotide Accession #NM_001204062.1
Protein Size (# AA)326
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