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Catalog No: OKEH03315
Size:96T
Price: $525.00
SKU
OKEH03315
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BMF ELISA Kit (Human) (OKEH03315) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for BMF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMF captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.5% (n = 20)
Mean Inter-assay CV%: <= 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-BMF Microplate96 Wells (12 x 8 Well strips)
BMF Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated BMF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.5 pg/mL
SpecificityNatural and recombinant Human Bcl-2-modifying factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBMF
Gene Full NameBcl2 modifying factor
Alias Symbolsbcl-2-modifying factor.
NCBI Gene Id90427
Protein NameQ96LC9
Description of TargetBcl-2-modifying factor
Uniprot IDhttps://www.uniprot.org/uniprot/Q96LC9
Protein Accession #NP_001003940.1
Nucleotide Accession #NM_001003940.1
Protein Size (# AA)184
Molecular Weight21 kDa
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