Human BLNK Antibody (Phospho-Tyr84) (OAAQ00171)

Catalog No: OAAQ00171

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 10 ng/mL of Phospho-BLNK (Tyr84) antibody BLNKY84-H4 (Abwiz Cat. #OAAQ00171) or Company B antibody at 2.5 ug/mL (manufacturer's recommended concentration).

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 ug/mL. Cat. #OAAQ00171.

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 ug/mL. Cat. #OAAQ00171.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 10 ng/mL of Phospho-BLNK (Tyr84) antibody BLNKY84-H4 (Abwiz Cat. #OAAQ00171) or Company B antibody at 2.5 ug/mL (manufacturer's recommended concentration).

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 ug/mL. Cat. #OAAQ00171.

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 ug/mL. Cat. #OAAQ00171.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: BLNKY84-H4
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr84 of human phospho BLNK
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Tyr84
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: Fu, C., et al. (1998) Immunity 9: 93-103.
  Goitsuka, R., et al. (1998) J. Immunol. 161: 5804-5808.
  Tsuji, S., et al. (2001) J. Exp. Med. 194: 529-539.

Target Info

• Gene Symbol: BLNK
• Gene Full Name: B cell linker
• Alias Symbols: bca, AGM4, BASH, LY57, SLP65, BLNK-S, SLP-65
• NCBI Gene Id: 29760
• Protein Name: B-cell linker protein
• Description of Target: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene.
• Uniprot ID: Q8WV28
• Protein Accession #: NP_001107566.1
• Nucleotide Accession #: NM_001114094.1