BLM Recombinant Protein (Mouse) (OPCA05207)

Catalog No: OPCA05207

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Product Format: Liquid or Lyophilized powder
• Additional Information: Species Specificity Detail: Mus musculus (Mouse)
• Reconstitution and Storage: -20°C or -80°C
• Formulation: Tris-base, 50% glycerol
• Purity: Greater than 90% as determined by SDS-PAGE.
• Peptide Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ
• Protein Sequence: INAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQ
• Storage Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Source: E.coli
• Protein Range: 684-859 aa
• Tag: N-terminal 10xHis-tagged and C-terminal MYC-tagged
• Reference: Point mutations causing Bloom's syndrome abolish ATPase and DNA helicase activities of the BLM protein. Bahr A., de Graeve F., Kedinger C., Chatton B. Oncogene 17:2565-2571(1998)

Target Info

• Gene Symbol: Blm
• Gene Full Name: Bloom syndrome, RecQ like helicase
• Alias Symbols: Bloom syndrome homolog;Bloom syndrome protein homolog;Bloom syndrome, RecQ helicase-like;mBLM;recQ helicase homolog.
• NCBI Gene Id: 12144
• Protein Name: Bloom syndrome protein homolog
• Description of Target: ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:9840919, PubMed:27010503). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity).
• Uniprot ID: O88700
• Protein Accession #: NP_001035992
• Nucleotide Accession #: NM_001042527
• Protein Size (# AA): Recombinant
• Molecular Weight: 24.7 kDa