BLM Antibody (Phospho-Thr99) (OAAF00142)

Catalog No: OAAF00142 (Formerly GWB-ASB326)

Size:100 ug

Western blot analysis of extracts from HepG2 cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human heart, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Bloom Syndrome (Phospho-Thr99) Antibody. The picture on the right is blocked with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: Bloom Syndrome Protein (Phospho-Thr99) antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500-1:1000
  IHC: 1:50-1:100
  IF: 1:100-1:500
  ELISA: 1:5000
• Reference: Ellis N.A., Cell 83:655-666(1995).
  Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
  Pichierri P., EMBO J. 23:3154-3163(2004).

Target Info

• Gene Symbol: BLM
• Gene Full Name: BLM RecQ like helicase
• Alias Symbols: Bloom syndrome protein;Bloom syndrome RecQ like helicase;Bloom syndrome, RecQ helicase-like;BS;DNA helicase, RecQ-like type 2;MGRISCE1;recQ protein-like 3;RECQ2;RECQL2;RECQL3.
• NCBI Gene Id: 641
• Protein Name: Bloom syndrome protein
• Description of Target: ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9388193, PubMed:24816114, PubMed:25901030). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (PubMed:20639533, PubMed:24257077, PubMed:25901030).
• Uniprot ID: P54132
• Protein Accession #: NP_000048.1
• Molecular Weight: 159 kDa