BIRC6 ELISA Kit (Human) (OKCD08620)

Catalog No: OKCD08620

Size:96WELLS

BIRC6 ELISA Kit (Human) (OKCD08620) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Baculoviral IAP Repeat Containing Protein 6 (BIRC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Baculoviral IAP Repeat Containing Protein 6 (BIRC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Baculoviral IAP Repeat Containing Protein 6 (BIRC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Baculoviral IAP Repeat Containing Protein 6 (BIRC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Baculoviral IAP Repeat Containing Protein 6 (BIRC6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Baculoviral IAP Repeat Containing Protein 6 (BIRC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BIRC6 Microplate: 96 Wells (12 x 8 Well strips)
  • BIRC6 Lyophilized Standard: 2
  • 100X BIRC6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.116ng/mL

Target Info

• Gene Symbol: BIRC6
• Gene Full Name: baculoviral IAP repeat containing 6
• Alias Symbols: APOLLON;baculoviral IAP repeat-containing protein 6;BIR repeat-containing ubiquitin-conjugating enzyme;BRUCE;RING-type E3 ubiquitin transferase BIRC6;Ubiquitin-conjugating BIR domain enzyme apollon;ubiquitin-conjugating BIR-domain enzyme apollon.
• NCBI Gene Id: 57448
• Protein Name: Baculoviral IAP repeat-containing protein 6
• Description of Target: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.
• Uniprot ID: Q9NR09
• Protein Accession #: NP_057336.3
• Nucleotide Accession #: NM_016252.3