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Datasheets/Manuals | Printable datasheet for BIRC5 Antibody (OAPB00092) (OAPB00092) |
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Predicted Species Reactivity | Bovine|Human|Mouse|Porcine|Rat |
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Product Format | Liquid. Survivin Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | Survivin antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Anti-Survivin antibody (2233) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human Survivin. The immunogen is located within the first 50 amino acids of Survivin. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-BIRC5 (OAPB00092) antibody is Catalog # OEPB02218 |
Application Info | Survivin antibody can be used for detection of survivin by Western blot at 0.5 to 1 ug/ml. An approximate 17 kDa band should be detected. (Optimal dilution should be determined by user) |
Reference | Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-21 ;Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC . Control of apoptosis and mitotic spindle checkpoint by survivin. Nature 1998;396:580-4;Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T. Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation. Proc Natl Acad Sci USA 1999;96:1457-62 (WD0600); |
Gene Symbol | BIRC5 |
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Gene Full Name | baculoviral IAP repeat containing 5 |
Alias Symbols | API4;apoptosis inhibitor 4;apoptosis inhibitor survivin;baculoviral IAP repeat-containing protein 5;EPR-1;survivin variant 3 alpha. |
NCBI Gene Id | 332 |
Protein Name | Baculoviral IAP repeat-containing protein 5|baculoviral IAP repeat-containing protein 5 isoform 1 [Homo sapiens] |
Description of Target | Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797). |
Uniprot ID | O15392 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001159 |
Nucleotide Accession # | NM_001012270.1; NM_001012271.1; NM_001168.2 |
Molecular Weight | Predicted: 17kD Observed: 17 kD |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BIRC5 Antibody (OAPB00092)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Porcine|Rat".
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How long will it take to receive "BIRC5 Antibody (OAPB00092)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "BIRC5 Antibody (OAPB00092)" provided in?
This item is provided in "Liquid. Survivin Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BIRC5 Antibody (OAPB00092)"?
This target may also be called "API4;apoptosis inhibitor 4;apoptosis inhibitor survivin;baculoviral IAP repeat-containing protein 5;EPR-1;survivin variant 3 alpha." in publications.
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What is the shipping cost for "BIRC5 Antibody (OAPB00092)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BIRC5 Antibody (OAPB00092)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BIRC5 Antibody (OAPB00092)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 17kD Observed: 17 kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BIRC5 Antibody (OAPB00092)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BIRC5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BIRC5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BIRC5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BIRC5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BIRC5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BIRC5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.