BIRC5 Antibody (OAAF00842)

Catalog No: OAAF00842 (Formerly GWB-ASC255)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Survivin Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Survivin.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Survivin antibody detects endogenous levels of total Survivin protein
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Alessandra Marconi, Stem Cells, Jan 2007; 25: 149 - 155.
  H Caldas, J. Med. Genet., Feb 2006; 43: 119 - 128.
  Koichi Asanuma, J. Immunol., Mar 2004; 172: 3922.

Target Info

• Gene Symbol: BIRC5
• Gene Full Name: baculoviral IAP repeat containing 5
• Alias Symbols: API4;apoptosis inhibitor 4;apoptosis inhibitor survivin;baculoviral IAP repeat-containing protein 5;EPR-1;survivin variant 3 alpha.
• NCBI Gene Id: 332
• Protein Name: Baculoviral IAP repeat-containing protein 5
• Description of Target: Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
• Uniprot ID: O15392
• Protein Accession #: NP_001012270.1
• Molecular Weight: 16 kDa