Biotinylated CCL7 Protein (OPCB00015)

Catalog No: OPCB00015

Size:2ug

Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through the Transwell bare filter at various concentrations of MCP-3. The response is expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Product Info

• Product Format: Lyophilized
• Conjugation: Biotin
• Application: CA
• Additional Information: Extinction Coefficient: 14,180 M-1 cm-1
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method
• Reconstitution and Storage: Spin tube prior to resuspending. Recommended at 100ug/mL in sterile water
  Shipping: Room Temp
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 1.4-2.6 nM determined by migration assay with cells expressing recombinant CCR2
  
• Protein Sequence: The protein sequence is: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKLKLGSGLNDIFEAQKIEWHE
• Source: E. coli
• Reference: 1. "Monocyte chemotactic protein-3"
  Menten P., Wuyts A., Van Damme J
  Eur. Cytokine Netw. 12:554-560 (2002)
  2. "Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines".
  Opdenakker G., Froyen G., Fiten P., Proost P., Van Damme J.
  Biochem. Biophys. Res. Commun. 191: 535–42 (1993)
  3. "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist."
  Blanpain C., Migeotte I., Lee B., Vakili J., Doranz B.J., Govaerts C., Vassart G., Doms R.W., Parmentier M.Blood 94:1899-1905 (1999)

Target Info

• Gene Symbol: CCL7
• Gene Full Name: chemokine (C-C motif) ligand 7
• Alias Symbols: FIC, MARC, MCP3, NC28, MCP-3, SCYA6, SCYA7
• NCBI Gene Id: 6354
• Description of Target: Monocyte chemotactic protein 3 (MCP3)(CCL7) is secreted by monocytes and certain tumer cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP3 binds and signals through several cell surface receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5.
• Uniprot ID: P80098
• Molecular Weight: 11.2kDa