Binuclease, Molecular Biology Grade (OPGG00003)

Catalog No: OPGG00003

Size:50 KU

Binuclease purity ≥ 95% as measured by SDS-PAGE

Product Info

• Product Format: Lyophilized
• Host: Yeast, Pichia pastoris
• Application: IA, Sample Prep for immunoassays
• Additional Information: Specific Activity is ≥1000 kU/mg protein. One unit of Binuclease will digest sonicated salmon sperm DNA to produce acid-soluble oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 minutes at pH 8.0 and 37°C.
  OD of 1.0 is 50 µg/mL for dsDNA, 33 µg/mL for ssDNA, and 40 µg/mL for RNA. Activity is >20 kU/mg of lyophilized powder.
  Active at pH 6~10, optimal pH=8.0. 1-10 mM Mg++ is an activator of the enzyme.
  Protease and Endotoxin free.
  Purity: >95%.
• Reconstitution and Storage: Reconstitute in 20 mM Tris-Cl (pH 8.0), 2 mM MgCl2, 20 mM NaCl, 50% glycerol. Lyophilized powder is stable for 3 years when stored below 4°C. Buffered aqueous glycerol solution is stable for 1 year when stored at -20°C.
• Protocol Information: Binuclease cleaves all forms of DNA and RNA, making it an ideal tool to eliminate nucleic acids (DNA and RNA) contamination in vaccine, antibody, cell therapy, and other biological products for therapeutic use to meet FDA guidelines that nucleic acid contamination should be limited to 100 pg per dose in the end-product.
  
  Binuclease reduces nucleic acid load and viscosity in cell lysates during purification to facilitate the filtration/ultrafiltration of solutions, reduce processing time, improve the separation of pellets and supernatants in centrifugations, and increase the efficiency of chromatographic purification, resulting in significant improvements on both yield and purity of cell-derived particles such as viruses, AAV vectors or inclusion bodies.
  
  Bioanalytical applications: Sample preparation for ELISA, chromatography or two dimensional electrophoresis (protein mapping), and footprint analysis to get improved resolution and increased recovery of samples.

Target Info

• Alias Symbols: NucA;binuclease;nuclease;endonuclease;Serratia marcescens binuclease;unrestricted endonuclease;
• Protein Name: Binuclease
• Uniprot ID: https://www.uniprot.org/uniprotkb/P13717/entry
• Protein Accession #: P13717
• Protein Size (# AA): 266
• Molecular Weight: 30 kDa