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BIM/BOD (IN2) Antibody (OASE00383)

  • Catalog#: OASE00383
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Gene Symbol:
BCL2L11
Official Gene Full Name:
BCL2-like 11 (apoptosis facilitator)
NCBI Gene Id:
10018
Swissprot Id:
O43521
Protein Accession #:
NP_619527.1
Replacement Item:
This antibody may replace item sc-11425 from Santa Cruz Biotechnology.
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB, ICC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express BCL2L11.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express BCL2L11.
Immunogen:
Internal central amino acids of human Bim
Predicted Species Reactivity:
Human, Mouse, Rat
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
1mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00383
Dilution:
1:400 (WB), 1:100 (ICC)
Additional Information:
Background Info: Detects a 23kDa protein corresponding to the molecular mass of Bim on SDS PAGE immunoblots.
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Scientific Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively.
Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
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Certificate of Analysis: 2.5 ug/mL was sufficient for detection of Bim in 20ug of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Target Reference:
1. O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
2. Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
Storage Buffer:
PBS with 0.02% sodium azide

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