Biglycan ELISA Kit (Human) (OKBB00620)

Catalog No: OKBB00620

Size:96 Wells

Human Biglycan ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Biglycan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Biglycan has been precoated onto 96-well plates. Standards(NS0, D38-K368) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Biglycan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Biglycan amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.62	2.91	5.38	1.52	3.07	6.34
  SD	0.06	0.157	0.264	0.062	0.209	0.33
  CV%	3.7	5.4	4.9	4.1	6.8	5.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Biglycan Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Biglycan Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Biglycan Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA) and tissue homogenates
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Biglycan
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D38-K368
• Description: For quantitative detection of human Biglycan in cell culture supernatants, serum and plasma(heparin, EDTA).

Target Info

• Gene Symbol: BGN
• Gene Full Name: biglycan
• Alias Symbols: BGN, Biglycan, Biglycan proteoglycan, Bone/cartilage proteoglycan I, Dermatan sulphate proteoglycan I, DSPG1, PG S1, PG-S1, PGI, PGS1_HUMAN, SLRR1A, Small leucine rich protein 1A
• NCBI Gene Id: 633
• Protein Name: Biglycan
• Description of Target: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.
• Uniprot ID: P21810
• Protein Accession #: NP_001702.1
• Nucleotide Accession #: NM_001711.5