Catalog No: OKEH03166
Size:96T
Price: $525.00
SKU
OKEH03166
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BID ELISA Kit (Rat) (OKEH03166) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bid has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Bid is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bid captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Bid Microplate96 Wells (12 x 8 Well strips)
Bid Lyophilized Standard2
100X Biotinylated Bid Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.157 ng/mL
SpecificityNatural and recombinant Rat BH3-interacting domain death agonist
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBid
Gene Full NameBH3 interacting domain death agonist
Alias Symbolsapoptotic death agonist BID;BH3-interacting domain death agonist;desmocollin type 4;p22 BID.
NCBI Gene Id64625
Protein NameQ9JLT6
Description of TargetBH3-interacting domain death agonist
Uniprot IDhttps://www.uniprot.org/uniprot/Q9JLT6
Protein Accession #NP_073175.1
Nucleotide Accession #NM_022684.1
Protein Size (# AA)196
Molecular Weight22 kDa
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