Catalog No: OKCD09294
Size:96WELLS
Price: $750.00
SKU
OKCD09294
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for BHLHB8 ELISA kit (Human) |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colormetric|Regular detection | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C, -20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.071ng/mL |
Gene Symbol | BHLHA15 |
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Gene Full Name | basic helix-loop-helix family member a15 |
Alias Symbols | basic helix-loop-helix domain containing, class B, 8;BHLHB8;class A basic helix-loop-helix protein 15;class B basic helix-loop-helix protein 8;class II bHLH protein MIST1;MIST1;MIST-1;muscle, intestine and stomach expression 1. |
NCBI Gene Id | 168620 |
Protein Name | Q7RTS1 |
Description of Target | Class A basic helix-loop-helix protein 15 |
Uniprot ID | https://www.uniprot.org/uniprot/Q7RTS1 |
Protein Accession # | NP_803238.1 |
Nucleotide Accession # | NM_177455.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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