BGLAP ELISA Kit (Rabbit) (OKCA00940)

Catalog No: OKCA00940

Size:96 Wells

Rabbit BGLAP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rabbit
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	82-93
  1:2	95	89-99
  1:4	94	90-90
  1:8	94	86-98

• ELISA Kit Principle: Aviva Systems Biology BGLAP ELISA Kit (Rabbit) (OKCA00940) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for BGLAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BGLAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BGLAP captured in well.
• ELISA Kit Range: 1.25 ng/mL-80 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92	90-94
  EDTA Plasma (n=4)	92	87-96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level BGLAP were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level BGLAP were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • BGLAP Microplate: 96 Wells (12 x 8 Well strips)
  • BGLAP Lyophilized Standard: 2
  • 100X Biotinylated BGLAP Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 0.31 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BGLAP
• Gene Full Name: Osteocalcin
• Protein Name: Osteocalcin
• Description of Target: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
• Uniprot ID: P39056
• Protein Accession #: XP_002715429.1
• Nucleotide Accession #: XM_002715383.3