Beta-APP42 ELISA Kit (Human) (OKEH00815)

Catalog No: OKEH00815

Size:96 Wells

Human Beta-APP42 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Beta-APP42 ELISA Kit (Human) (OKEH00815) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Beta-APP42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Beta-APP42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Beta-APP42 captured in well.
• ELISA Kit Range: 7.8 - 500pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.7% (n = 20)
  Mean Inter-assay CV%: < 6.9% (n = 20)
• ELISA Kit Component:
  • Beta-APP42 Microplate: 96 Wells (12 x 8 Well strips)
  • Beta-APP42 Lyophilized Standard: 2
  • 100X Biotinylated Beta-APP42 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 3.12 pg/mL
• Specificity: Natural and recombinant Human Beta-amyloid protein 42
• Publications:

  Oxidative Stress in Alzheimer's Disease: In Vitro Therapeutic Effect of Amniotic Fluid Stem Cells Extracellular Vesicles. Oxid Med Cell Longev. 2020, 2785343 (2020). 33193997

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Beta-APP42
• Alias Symbols: Beta-amyloid protein 42
• Protein Name: Beta-amyloid protein 42
• Description of Target: Human form of the predominant amyloid β-peptide found in plaques associated with Alzheimer's disease. Leads to a large shift in the bax/bcl-2 ratio in favour of pro-apoptotic bax. Neurotoxic.