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Catalog No: OKIA00134 (Formerly GWB-374Z41)
Size:1x96WELLPLATE
Price: $509.00
SKU
OKIA00134
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration90 Minutes
ELISA Kit PrincipleAviva Systems Biology beta-2 Microglobulin ELISA Kit (Rat) (OKIA00134) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Beta-2-microglobulin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Beta-2-microglobulin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Beta-2-microglobulin captured in the well.
ELISA Kit Range0.156 ng/ml - 5 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Rat beta-2 Microglobulin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat beta-2 Microglobulin Calibrator1
Additional InformationIntended Use: The Beta 2-Microglobulin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Beta 2-Microglobulin in biological fluids of Rat.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Beta 2-Microglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT B2M ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat B2M.
7. RAT B2M CALIBRATOR
One vial containing a Rat Beta 2-Microglobulin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.835 ng/ml
Predicted Homology Based on Immunogen SequenceRat
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolB2M
Gene Full Namebeta-2 microglobulin
Alias SymbolsBeta-2-microglobulin, B2M
NCBI Gene Id24223
Protein NameBeta-2-microglobulin
Description of TargetComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Uniprot IDP07151
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