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Datasheets/Manuals | Printable datasheet for BECN1 Antibody (OAPB00348) (OAPB00348) |
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Predicted Species Reactivity | Bovine|Human|Mouse|Porcine|Rat |
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Product Format | Liquid. Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | Anti-Beclin-1 antibody (3613) was raised against a peptide corresponding to 17 amino acids near the center of human Beclin-1. The immunogen is located between 40-90 amino acids of Beclin-1. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-BECN1 (OAPB00348) antibody is Catalog # OEPB02438 |
Application Info | Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5-2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. Optimal dilutions for each application to be determined by the researcher. |
Reference | Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.; Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.; Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.; Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91. |
Gene Symbol | BECN1 |
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Gene Full Name | beclin 1 |
Alias Symbols | ATG6;ATG6 autophagy related 6 homolog;beclin 1 (coiled-coil, moesin-like BCL2-interacting protein);beclin 1, autophagy related;beclin1;beclin-1;coiled-coil myosin-like BCL2-interacting protein;Protein GT197;testis secretory sperm-binding protein Li 215e;VPS30. |
NCBI Gene Id | 8678 |
Protein Name | Beclin 1, autophagy related [Homo sapiens]|Beclin-1 |
Description of Target | Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. |
Uniprot ID | Q14457 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/AAH10276 |
Nucleotide Accession # | NM_003766.3; XM_005257758.2 |
Molecular Weight | Predicted: 52 kD Observed: 60 kD |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BECN1 Antibody (OAPB00348)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Porcine|Rat".
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How long will it take to receive "BECN1 Antibody (OAPB00348)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "BECN1 Antibody (OAPB00348)" provided in?
This item is provided in "Liquid. Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BECN1 Antibody (OAPB00348)"?
This target may also be called "ATG6;ATG6 autophagy related 6 homolog;beclin 1 (coiled-coil, moesin-like BCL2-interacting protein);beclin 1, autophagy related;beclin1;beclin-1;coiled-coil myosin-like BCL2-interacting protein;Protein GT197;testis secretory sperm-binding protein Li 215e;VPS30." in publications.
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What is the shipping cost for "BECN1 Antibody (OAPB00348)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BECN1 Antibody (OAPB00348)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BECN1 Antibody (OAPB00348)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 52 kD Observed: 60 kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BECN1 Antibody (OAPB00348)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BECN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BECN1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BECN1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BECN1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BECN1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BECN1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.