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BDNF ELISA Kit (Mouse) : 96 Wells (OKEH00022)

96 Wells
$529.00
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Predicted Species Reactivity:
Mouse
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
BDNF
Official Gene Full Name:
brain derived neurotrophic factor
Alias Symbols:
BDNF, Brain-derived neurotrophic factor
NCBI Gene Id:
12064
Protein Name:
Brain-derived neurotrophic factor
Description of Target:
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
Swissprot Id:
P21237
Protein Accession #:
NP_001041604
Nucleotide Accession #:
NM_001048139
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.08 ng/mL
Kit Range:
0.156 - 10ng/ml
Kit Reproducibility:
Mean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology BDNF ELISA Kit (Mouse) (OKEH00022) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDNF captured in well.
Kit Component:
ComponentAmount
BDNF Microplate96 Wells (12 x 8 Well strips)
BDNF Lyophilized Standard2
100X Biotinylated BDNF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express BDNF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express BDNF.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Mouse Brain-derived neurotrophic factor
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
28 kDa
Protein Size (# AA):
249

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153/06/2018 21:42
  • Overall Experience:
  • Quality:
ELISA in milk samples

It worked great and we could detect BDNF in milk samples collected from mouse

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