Catalog No: OKEH00022
Size:96 Wells
Price: $475.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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BDNF ELISA Kit (Mouse) : 96 Wells (OKEH00022)

80% of 100
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00022
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BDNF ELISA Kit (Mouse) (OKEH00022) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BDNF captured in well.
ELISA Kit Range0.156 - 10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
BDNF Microplate96 Wells (12 x 8 Well strips)
BDNF Lyophilized Standard2
100X Biotinylated BDNF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.08 ng/mL
SpecificityNatural and recombinant Mouse Brain-derived neurotrophic factor

Maternal Nicotinamide Riboside Enhances Postpartum Weight Loss, Juvenile Offspring Development, and Neurogenesis of Adult Offspring. Cell Rep. 26, 969-983.e4 (2019). 30673618

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBDNF
Gene Full Namebrain derived neurotrophic factor
Alias SymbolsBDNF, Brain-derived neurotrophic factor
NCBI Gene Id12064
Protein NameBrain-derived neurotrophic factor
Description of TargetDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
Uniprot IDP21237
Protein Accession #NP_001041604
Nucleotide Accession #NM_001048139
Protein Size (# AA)249
Molecular Weight28 kDa
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