BDNF ELISA Kit (Monkey) (OKCA01604)

Catalog No: OKCA01604

Size:96 Wells

Monkey BDNF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Monkey
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology BDNF ELISA Kit (Monkey) (OKCA01604) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BDNF captured in the well.
• ELISA Kit Range: 0.78 - 50 ng/mL
• ELISA Kit Component:
  • BDNF Microplate: 96 Wells (12 x 8 Well strips)
  • BDNF Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated BDNF Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.195 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: BDNF
• Gene Full Name: Brain-derived neurotrophic factor
• Alias Symbols: MGC34632, neurotrophin
• Protein Name: Brain-derived neurotrophic factor
• Description of Target: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.
• Uniprot ID: Q06225
• Protein Accession #: XP_014970085.1
• Nucleotide Accession #: XM_015114599.1