BDNF ELISA Kit (Human) : 96 Wells (OKAG00197)

Catalog No: OKAG00197 (Formerly GWB-SKR197)

Size:1 plate: 12 x 8-Well Microstrips

BDNF ELISA Kit (Human) (OKAG00197) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Product Format: 1 x 96-Well Plate or 12 x 8-Well Strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: The Aviva Human BDNF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hBDNF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human BDNF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
• ELISA Kit Range: 47-1500 pg/mL
• ELISA Kit Component:
  • Capture Antibody Coated Microplate: 96 Wells (12 x 8)
  • Ready-to-Use Streptavidin-HRP: 12 mL
  • Ready-to-Use Substrate: 12 mL
  • Stop Solution: 12 mL
  • Wash Buffer (15x): 50 mL
  • Protein Standard Diluent: 12 mL
  • Sample Diluent: 12 mL
  • Detection Antibody Diluent: 12 mL
  • Biotin-Labeled Detection Antibody: Lyophilized
  • Protein Standard: Lyophilized (11 ng)
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Cell lysates, sera and plasma
• Sensitivity: 47 pg/mL
• Specificity: The Human BDNF ELISA is capable of recognizing both recombinant and naturally produced Human BDNF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
  • Human: β-NGF, GDNF, NT-3, NT-4
• Publications:

  Male rats exhibit higher pro-BDNF, c-Fos and dendritic tree changes after chronic acoustic stress. Biosci Trends. 13, 546-555 (2020). 31956226

• Assay Info: Quantitative Colorimentric Sandwich ELISA

Target Info

• Gene Symbol: BDNF
• Gene Full Name: brain-derived neurotrophic factor
• Alias Symbols: ANON2, BULN2
• NCBI Gene Id: 627
• Protein Name: Brain-derived neurotrophic factor
• Description of Target: This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
• Uniprot ID: P23560
• Protein Accession #: NP_001137277.1
• Nucleotide Accession #: NM_001143805.1