Catalog No: OKAG00197 (Formerly GWB-SKR197)
Size:1 plate: 12 x 8-Well Microstrips
Price: $475.00
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleThe Aviva Human BDNF ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hBDNF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human BDNF cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range47-1500 pg/mL
ELISA Kit Component
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (11 ng)
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, sera and plasma
Sensitivity47 pg/mL
SpecificityThe Human BDNF ELISA is capable of recognizing both recombinant and naturally produced Human BDNF proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Human: β-NGF, GDNF, NT-3, NT-4

Male rats exhibit higher pro-BDNF, c-Fos and dendritic tree changes after chronic acoustic stress. Biosci Trends. 13, 546-555 (2020). 31956226

Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolBDNF
Gene Full Namebrain-derived neurotrophic factor
Alias SymbolsANON2, BULN2
NCBI Gene Id627
Protein NameBrain-derived neurotrophic factor
Description of TargetThis gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
Uniprot IDP23560
Protein Accession #NP_001137277.1
Nucleotide Accession #NM_001143805.1
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