Catalog No: OKCD03317
Size:96WELLS
Price: $800.00
SKU
OKCD03317
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BDNF Chemi-Luminescent ELISA Kit (Human) (OKCD03317)

Datasheets/ManualsPrintable datasheet for BDNF Chemi-Luminescent ELISA Kit (Human) (OKCD03317)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)94-102%99-105%93-105%91-103%
EDTA Plasma (n=5)89-97%83-95%93-105%80-97%
Heparin Plasma (n=5)91-104%86-94%79-104%98-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Brain Derived Neurotrophic Factor (BDNF) level in the sample or standard.
ELISA Kit Range27.4-20,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)81-9187
EDTA Plasma (n=5)85-10190
Heparin Plasma (n=5)92-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
BDNF Microplate96 Wells (12 x 8 Well strips)
BDNF Lyophilized Standard2
100X BDNF Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity27.4 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBDNF
Gene Full Namebrain derived neurotrophic factor
Alias Symbolsabrineurin;ANON2;brain-derived neurotrophic factor;BULN2;neurotrophin.
NCBI Gene Id627
Protein NameBrain-derived neurotrophic factor
Description of TargetDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Uniprot IDP23560
Protein Accession #NP_001137277
Nucleotide Accession #NM_001143805
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