BDNF Antibody (OABB01538)

Catalog No: OABB01538

Size:100UG

Anti-BDNF antibody
All lanes: Anti-BDNF(OABB01538) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 28 kDa
Observed bind size: 28 kDa

Anti-BDNF antibody
IHC(P): Mouse Brain Tissue

Anti-BDNF antibody
IHC(P): Rat Brain Tissue

Product Info

• Tested Species Reactivity: Mouse, Rat
• Predicted Species Reactivity: Bovine, Dog, Horse, Human, Monkey, Mouse, Rabbit, Rat
• Product Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry, Immunocytochemistry, Immunohistochemistry, Western blot
• Additional Information: Notes: WB: The detection limit for BDNF is approximately 0.25ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Immunogen: Anti-BDNF Picoband™ Antibody (PB9075) was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Mouse, Rat: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: Human: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
  Immunocytochemistry: 0.5-1 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Storage Buffer: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: Bdnf
• Gene Full Name: brain derived neurotrophic factor
• Alias Symbols: brain-derived neurotrophic factor.
• NCBI Gene Id: 12064
• Protein Name: P21237
• Description of Target: Brain-derived neurotrophic factor
• Uniprot ID: https://www.uniprot.org/uniprot/P21237
• Molecular Weight: 28123 MW