BCL6 ELISA Kit (Human) (OKCA01120)

Catalog No: OKCA01120

Size:96 Wells

Human BCL6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	98	94-103
  1:2	100	97-104
  1:4	86	83-89
  1:8	98	94-103

• ELISA Kit Principle: Aviva Systems Biology BCL6 ELISA Kit (Human) (OKCA01120) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BCL6 captured in the well.
• ELISA Kit Range: 18.75 - 1,200 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	85	81-89
  EDTA Plasma (n=4)	87	83-90

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01120 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01120 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • BCL6 Microplate: 96 Wells (12 x 8 Well strips)
  • BCL6 Lyophilized Standard: 2 x 1.2 ng
  • 100X Biotinylated BCL6 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 4.68 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: BCL6
• Gene Full Name: B-cell CLL/lymphoma 6
• Alias Symbols: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
• NCBI Gene Id: 604
• Protein Name: B-cell lymphoma 6 protein
• Description of Target: Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.1
• Uniprot ID: P41182
• Protein Accession #: NP_001124317.1
• Nucleotide Accession #: NM_001130845.1