BCL6 Antibody (Phospho-Ser333) (OAAF00528)

Catalog No: OAAF00528

Size:100 ug

Western blot analysis of extracts from COLO205 cells treated with insulin 0.01U/ml 15', using Bcl-6 (Phospho-Ser333) Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Additional Information: Modification Sites: H: S333 M: S334 R: S334
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Bcl-6 around the phosphorylation site of Ser333.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Concentration: 1 mg/ml
• Specificity: Bcl-6 (Phospho-Ser333) antibody detects endogenous levels of Bcl-6 only when phosphorylated at serine 333.
• Application Info: WB: 1:500~1:1000
  ELISA: 1:1000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: BCL6
• Gene Full Name: BCL6 transcription repressor
• Alias Symbols: B cell CLL/lymphoma 6;B-cell lymphoma 5 protein;B-cell lymphoma 6 protein;B-cell lymphoma 6 protein transcript;BCL5;BCL-5;BCL-6;BCL6A;cys-his2 zinc finger transcription factor;LAZ3;lymphoma-associated zinc finger gene on chromosome 3;protein LAZ-3;ZBTB27;zinc finger and BTB domain-containing protein 27;zinc finger protein 51;zinc finger transcription factor BCL6S;ZNF51.
• NCBI Gene Id: 604
• Protein Name: B-cell lymphoma 6 protein
• Description of Target: Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
• Uniprot ID: P41182
• Protein Accession #: NP_001124317.1
• Molecular Weight: 78 kDa