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Datasheets/Manuals | Printable datasheet for OAAB06867 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid PBS with 0.09% (W/V) sodium azide. |
Clonality | Monoclonal |
Clone | 305CT16.3.3 |
Isotype | IgG1,k |
Host | Mouse |
Application | WB |
Additional Information | Function: Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation. Cellular Location: Nucleus Tissue Location: Expressed in germinal center T and B-cells and in primary immature dendritic cells |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This BCL6 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 676-704 amino acids from the C-terminal region of human BCL6. |
Purification | This antibody is purified through a protein G column, followed by dialysis against PBS. |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:100~250 |
Gene Symbol | BCL6 |
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Alias Symbols | BCL5, LAZ3, BCL6A, ZNF51, ZBTB27 |
NCBI Gene Id | 604 |
Protein Name | B-cell lymphoma 6 protein |
Description of Target | The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. |
Uniprot ID | P41182 |
Protein Accession # | NP_001124317.1 |
Molecular Weight | 79 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BCL6 Antibody - C-terminal region (OAAB06867)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "BCL6 Antibody - C-terminal region (OAAB06867)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "BCL6 Antibody - C-terminal region (OAAB06867)" provided in?
This item is provided in "Liquid PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BCL6 Antibody - C-terminal region (OAAB06867)"?
This target may also be called "BCL5, LAZ3, BCL6A, ZNF51, ZBTB27" in publications.
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What is the shipping cost for "BCL6 Antibody - C-terminal region (OAAB06867)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BCL6 Antibody - C-terminal region (OAAB06867)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BCL6 Antibody - C-terminal region (OAAB06867)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "79 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BCL6 Antibody - C-terminal region (OAAB06867)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BCL6"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BCL6"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BCL6"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BCL6"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BCL6"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BCL6"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.