BCL6 Antibody - C-terminal region (OAAB04379)

Catalog No: OAAB04379

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Western blot analysis of Bcl-6 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). BCL6 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lymph tissue with Bcl-6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Bcl-6 Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
  Cellular Location: Nucleus
  Tissue Location: Expressed in germinal center T- and B-cells and in primary immature dendritic cells.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Bcl-6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the C-terminal region of human Bcl-6.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Bcl-6 Antibody (C-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: BCL6
• Alias Symbols: BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
• NCBI Gene Id: 604
• Protein Name: B-cell lymphoma 6 protein
• Description of Target: BCL6 is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors.
• Uniprot ID: P41182
• Protein Accession #: NP_001124317.1
• Molecular Weight: 79 kDa