BCL3 ELISA Kit (Human) : 96 Wells (OKEH01245)

Catalog No: OKEH01245

Size:96T

Human BCL3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology BCL3 ELISA Kit (Human) (OKEH01245) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 9.2% (n = 20)
• ELISA Kit Component:
  • BCL3 Microplate: 96 Wells (12 x 8 Well strips)
  • BCL3 Lyophilized Standard: 2
  • 100X Biotinylated BCL3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.056 ng/mL
• Specificity: Natural and recombinant Human B-cell lymphoma 3 protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BCL3
• Gene Full Name: BCL3 transcription coactivator
• Alias Symbols: B cell CLL/lymphoma 3;B-cell leukemia/lymphoma 3;B-cell lymphoma 3 protein;B-cell lymphoma 3-encoded protein;BCL-3;BCL4;chronic lymphatic leukemia protein;D19S37;proto-oncogene BCL3.
• NCBI Gene Id: 602
• Protein Name: P20749
• Description of Target: B-cell lymphoma 3 protein
• Uniprot ID: https://www.uniprot.org/uniprot/P20749
• Protein Accession #: NP_005169.2
• Nucleotide Accession #: NM_005178.4
• Protein Size (# AA): 454
• Molecular Weight: 48 kDa