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Catalog No: OKWB00208
Size:96T
Price: $450.00
SKU
OKWB00208
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Datasheets/ManualsPrintable datasheet for BCL2L12 ELISA Kit (Human) (OKWB00208)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-102%85-105%85-105%85-105%
EDTA Plasma (n=5)87-101%91-98%86-101%91-95%
Heparin Plasma (n=5)82-100%82-90%87-100%82-99%
ELISA Kit PrincipleAviva Systems Biology BCL2L12 ELISA Kit (Human) (OKWB00208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL2L12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL2L12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL2L12 captured in the well.
ELISA Kit Range0.313-20ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)88-10192
EDTA Plasma (n=5)94-10098
Heparin Plasma (n=5)85-10297
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
BCL2L12 Microplate96 Wells (12 x 8 Well strips)
BCL2L12 Lyophilized Standard2 x 20 ng
100X BCL2L12 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolBCL2L12
Gene Full NameBCL2 like 12
Alias SymbolsBcl-2 related proline-rich protein;BCL2-like 12 (proline rich);bcl-2-like protein 12;Bcl-2-related proline-rich protein.
NCBI Gene Id83596
Protein NameBcl-2-like protein 12
Uniprot IDQ9HB09
Protein Accession #NP_001035758
Nucleotide Accession #NM_001040668
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