BCL2L11 ELISA Kit (Human) (OKCD02093)

Catalog No: OKCD02093 (Formerly GWB-KBBMO2)

Size:96WELLS

Human BCL2L11 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Like Protein 11 (BCL2L11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Like Protein 11 (BCL2L11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Like Protein 11 (BCL2L11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 Like Protein 11 (BCL2L11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Like Protein 11 (BCL2L11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Like Protein 11 (BCL2L11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BCL2L11 Microplate: 96 Wells (12 x 8 Well Strips)
  • BCL2L11 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated BCL2L11 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2 Like Protein 11 (BCL2L11). No significant cross-reactivity or interference between Bcl2 Like Protein 11 (BCL2L11) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BCL2L11
• Gene Full Name: BCL2 like 11
• Alias Symbols: BAM;bcl-2 interacting mediator of cell death;bcl-2 interacting protein Bim;Bcl2-interacting mediator of cell death;BCL2-like 11 (apoptosis facilitator);bcl-2-like protein 11;bcl-2-related ovarian death agonist;BIM;BOD.
• NCBI Gene Id: 10018
• Protein Name: Bcl-2-like protein 11
• Description of Target: Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
• Uniprot ID: O43521
• Protein Accession #: NP_001191035.1
• Nucleotide Accession #: NM_001204106.1
• Protein Size (# AA): 198