BCL2L10 Antibody (OAGA00927)

Catalog No: OAGA00927

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
OAGA00927 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BCL2L10(OAGA00927) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using BCL2L10(OAGA00927) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HepG2, Molt-4, Raji
  Cellular Localization: Mitochondrion, Nucleus membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 60 and 153 of BCL2L10 (Uniprot ID#Q9HD36)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: BCL2L10
• Gene Full Name: Bcl-2-like protein 10
• Alias Symbols: Boo, Diva, BCL-B, bcl2-L-10
• NCBI Gene Id: 10017
• Protein Name: Bcl-2-like protein 10
• Uniprot ID: Q9HD36
• Protein Accession #: NP_065129.1
• Molecular Weight: 23 kDa