BCL2A1 Antibody (OAPB00431)

Catalog No: OAPB00431

Size:100UG

Western blot analysis of Bfl-1 in human kidney tissue lysate with Bfl-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse
• Product Format: Liquid. Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Positive Samples: Human kidney tissue lysate, mouse kidney tissue lysate, human lung tissue lysate
• Reconstitution and Storage: Bfl-1 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies, care should be taken to avoid repeated freeze/thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Bfl-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Bfl-1. The immunogen is located within the last 50 amino acids of Bfl-1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Bovine (92%)
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BCL2A1 (OAPB00431) antibody is Catalog # OEPB02532
• Specificity: At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
• Application Info: WB: 1 ug/mL
  IHC-P: 10 ug/mL
  IF: 20 ug/mL
• Reference: Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.;Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.;Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-kappaB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.;Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem.2000; 275:11092-9

Target Info

• Gene Symbol: BCL2A1
• Gene Full Name: BCL2 related protein A1
• Alias Symbols: ACC1;ACC-1;ACC2;ACC-2;bcl2-L-5;BCL2L5;bcl-2-like protein 5;bcl-2-related protein A1;BFL1;GRS;HBPA1;hematopoietic BCL2-related protein A1;hemopoietic-specific early response protein;protein BFL-1;Protein GRS.
• NCBI Gene Id: 597
• Protein Name: Bcl-2-related protein A1|bcl-2-related protein A1 isoform 1 [Homo sapiens]
• Description of Target: Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).
• Uniprot ID: Q16548
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004040
• Nucleotide Accession #: NM_001114735.1; NM_004049.3
• Molecular Weight: Predicted: 19 kDa Observed: 20 kDa