Catalog No: OKCD06802
Size:96 Wells
Price: $650.00
SKU
OKCD06802
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BCL2 ELISA Kit (Rat) (OKCD06802) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL2 captured in the well.
ELISA Kit Range78-5,000pg/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level BCL2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level BCL2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
BCL2 Microplate96 Wells (12 x 8 Well strips)
BCL2 Lyophilized Standard2
100X BCL2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 28pg/mL
Gene SymbolBCL2
Gene Full NameBCL2, apoptosis regulator
Alias SymbolsBcl-2
NCBI Gene Id24224
Protein Nameapoptosis regulator Bcl-2
Description of Targetan anti-apoptotic protein; involved in inhibiting cell death in many different cell types.
Swissprot IdP49950
Protein Accession #NP_058689.1
Nucleotide Accession #NM_016993.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express BCL2.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express BCL2.
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