Catalog No: OKEH03217
Size:96 Wells
Price: $525.00
SKU
OKEH03217
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BCL2 ELISA Kit (Mouse) (OKEH03217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bcl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Bcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bcl2 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Bcl2 Microplate96 Wells (12 x 8 Well strips)
Bcl2 Lyophilized Standard2
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
100X Biotinylated Bcl2 Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Mouse Apoptosis regulator Bcl-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBCL2
Gene Full NameB cell leukemia/lymphoma 2
Alias SymbolsBcl-, Bcl-2, AW986256, C430015F12Rik, D630044D05Rik, D830018M01Rik
NCBI Gene Id12043
Protein NameApoptosis regulator Bcl-2
Description of TargetSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Swissprot IdP10417
Protein Accession #NP_033871.2
Nucleotide Accession #NM_009741.5
Protein Size (# AA)236
Molecular Weight26 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Bcl2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Bcl2.
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