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Catalog No: OKEH02656
Size:96T
Price: $475.00
SKU
OKEH02656
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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BCL2 ELISA Kit (Human) : 96 Wells (OKEH02656)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02656
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BCL2 ELISA Kit (Human) (OKEH02656) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for BCL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 6.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-BCL2 Microplate96 Wells (12 x 8 Well strips)
BCL2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated BCL2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity30 pg/mL
SpecificityNatural and recombinant Human Apoptosis regulator Bcl-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBCL2
Gene Full NameBCL2 apoptosis regulator
Alias Symbolsapoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;PPP1R50;protein phosphatase 1, regulatory subunit 50.
NCBI Gene Id596
Protein NameP10415
Description of TargetApoptosis regulator Bcl-2
Uniprot IDhttps://www.uniprot.org/uniprot/P10415
Protein Accession #NP_000624.2
Nucleotide Accession #NM_000633.2
Protein Size (# AA)239
Molecular Weight26 kDa
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