BCL11A Protein Lysate (OCOA08462)

Catalog No: OCOA08462

Size:20UG

Product Info

• Predicted Species Reactivity: Homo sapiens
• Product Format: Lyophilized
• Host: HEK293T
• Additional Information: Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
  Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
• Reconstitution and Storage: The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
• Tag: C-MYC/DDK
• Description: BCL11A HEK293T cell transient overexpression lysate (as WB positive control)

Target Info

• Gene Symbol: BCL11A
• Gene Full Name: BAF chromatin remodeling complex subunit BCL11A
• Alias Symbols: B cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A (zinc finger protein);B-cell lymphoma/leukemia 11A;BCL11A B-cell CLL/lymphoma 11A (zinc finger protein);BCL11A, BAF complex component;BCL11A-L;BCL11a-M;BCL11A-S;BCL11A-XL;C2H2-type zinc finger protein;COUP-TF-interacting protein 1;CTIP1;DILOS;ecotropic viral integration site 9 homolog;ecotropic viral integration site 9 protein homolog;EVI9;HBFQTL5;SMARCM1;zinc finger protein 856;ZNF856.
• NCBI Gene Id: 53335
• Protein Name: D9YZW0, NM_022893, NP_075044, Q9H165
• Description of Target: B-cell CLL/lymphoma 11A (Zinc finger protein), isoform CRA_b, B-cell lymphoma/leukemia 11A, B-cell lymphoma/leukemia 11A isoform 1 [Homo sapiens], Homo sapiens B cell CLL/lymphoma 11A (BCL11A), transcript variant 1, mRNA
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H165
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_075044
• Nucleotide Accession #: https://www.ncbi.nlm.nih.gov/nuccore/NM_022893
• Molecular Weight: 91 kDa