BCL11A ELISA Kit (Human) (OKEH06863)

Catalog No: OKEH06863

Size:96T

Human BCL11A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology BCL11A ELISA Kit (Human) (OKEH06863) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL11A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL11A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL11A captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 106%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.0%
  Inter-Assay CV <8.3%
• ELISA Kit Component:
  • BCL11A Microplate: 96 Wells (12 x 8 Well strips)
  • BCL11A Lyophilized Standard: 2 x 20 ng
  • 100X BCL11A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Human B-cell lymphoma/leukemia 11A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BCL11A
• Gene Full Name: BAF chromatin remodeling complex subunit BCL11A
• Alias Symbols: B cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A (zinc finger protein);B-cell lymphoma/leukemia 11A;BCL11A B-cell CLL/lymphoma 11A (zinc finger protein);BCL11A, BAF complex component;BCL11A-L;BCL11a-M;BCL11A-S;BCL11A-XL;C2H2-type zinc finger protein;COUP-TF-interacting protein 1;CTIP1;DILOS;ecotropic viral integration site 9 homolog;ecotropic viral integration site 9 protein homolog;EVI9;HBFQTL5;SMARCM1;zinc finger protein 856;ZNF856.
• NCBI Gene Id: 53335
• Protein Name: Q9H165
• Description of Target: B-cell lymphoma/leukemia 11A
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H165
• Protein Accession #: NP_060484
• Nucleotide Accession #: NM_018014