BCL11A ELISA Kit (Human) (OKCD00942)

Catalog No: OKCD00942 (Formerly GWB-KBBFP6)

Size:96WELLS

Human BCL11A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 11A (Bcl11A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 11A (Bcl11A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of B-Cell CLL/Lymphoma 11A (Bcl11A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell CLL/Lymphoma 11A (Bcl11A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BCL11A Microplate: 96 Wells (12 x 8 Well Strips)
  • BCL11A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated BCL11A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.109 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of B-Cell CLL/Lymphoma 11A (Bcl11A). No significant cross-reactivity or interference between B-Cell CLL/Lymphoma 11A (Bcl11A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BCL11A
• Gene Full Name: BAF chromatin remodeling complex subunit BCL11A
• Alias Symbols: B cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A;B-cell CLL/lymphoma 11A (zinc finger protein);B-cell lymphoma/leukemia 11A;BCL11A B-cell CLL/lymphoma 11A (zinc finger protein);BCL11A, BAF complex component;BCL11A-L;BCL11a-M;BCL11A-S;BCL11A-XL;C2H2-type zinc finger protein;COUP-TF-interacting protein 1;CTIP1;DILOS;ecotropic viral integration site 9 homolog;ecotropic viral integration site 9 protein homolog;EVI9;HBFQTL5;SMARCM1;zinc finger protein 856;ZNF856.
• NCBI Gene Id: 53335
• Protein Name: B-cell lymphoma/leukemia 11A
• Description of Target: Transcription factor associated with the BAF SWI/SNF chromatin remodeling complex (By similarity). Repressor of fetal hemoglobin (HbF) level (PubMed:26375765). Involved in brain development (PubMed:27453576). Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1 (By similarity).
• Uniprot ID: Q9H165
• Protein Accession #: NP_060484
• Nucleotide Accession #: NM_018014.3
• Protein Size (# AA): 835