Catalog No: OKEH04973
Size:96T
Price: $625.00
SKU
OKEH04973
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology BCL10 ELISA Kit (Human) (OKEH04973) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL10 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 108% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <7.2%, n = 20 Mean Inter-Assay Precision - <8.5%, n = 20 | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 39.4 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human B-cell lymphoma/leukemia 10 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | BCL10 |
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Gene Full Name | BCL10 immune signaling adaptor |
Alias Symbols | B cell CLL/lymphoma 10;B-cell CLL/lymphoma 10;B-cell lymphoma/leukemia 10;CARD containing molecule enhancing NF-kB;CARD-containing apoptotic signaling protein;CARD-containing molecule enhancing NF-kappa-B;CARD-containing proapoptotic protein;CARD-like apoptotic protein;CARMEN;caspase-recruiting domain-containing protein;cCARMEN;c-E10;CED-3/ICH-1 prodomain homologous E10-like regulator;cellular homolog of vCARMEN;cellular-E10;CIPER;CLAP;hCLAP;IMD37;mammalian CARD-containing adapter molecule E10;mE10. |
NCBI Gene Id | 8915 |
Protein Name | O95999 |
Description of Target | B-cell lymphoma/leukemia 10 |
Uniprot ID | https://www.uniprot.org/uniprot/O95999 |
Protein Accession # | NP_003912.1 |
Nucleotide Accession # | NM_003921.4 |
Protein Size (# AA) | 233 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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