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Datasheets/Manuals | Printable datasheet for anti-BCL2 antibody OAEC00063 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | IHC |
Reconstitution and Storage | Store at -20C/1 year |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H). |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation |
Concentration | 1 mg/ml |
Specificity | BCL-2 (phospho-Thr56) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Info | IHC: 1:50-1:100 |
Reference | Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478. |
Gene Symbol | BCL2 |
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Gene Full Name | Apoptosis regulator Bcl-2 |
Alias Symbols | Bcl-2, PPP1R50 |
NCBI Gene Id | 596 |
Protein Name | Apoptosis regulator Bcl-2 |
Uniprot ID | P10415 |
Protein Accession # | NP_000624.2 |
Molecular Weight | 26 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
This target may also be called "Bcl-2, PPP1R50" in publications.
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What is the shipping cost for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "26 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BCL-2 Antibody (Phospho-Thr56) (OAEC00063)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BCL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BCL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BCL2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BCL2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BCL2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BCL2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.