Bcl-2 Antibody (OAPB00242)

Catalog No: OAPB00242 (Formerly GWB-585B39)

Size:100UG

Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.

Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 μg/mL.

Immunofluorescence of Bcl-2 in Human Kidney cells with Bcl-2 antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Human|Mouse|Rat
• Product Format: Liquid PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: Bcl-2antibody can be stored at 4°C, for three months and -20°C stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-Bcl-2 antibody (3335) was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Bovine (100%), Rat (100%), Chicken (93%)
• Concentration: Varies by lot. se
• Blocking Peptide: For anti-Bcl-2 antibody is Catalog # OEPB02336
• Application Info: WB: 1-2 ug/mL
  IHC-P: 2 ug/mL
  IF: 10 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and mouse samples ; Immunofluorescence in human and mouse samples.
• Reference: Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.;
  Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.;
  Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.;
  Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
• Publications:

  Age-Related Gliosis Promotes Central Nervous System Lymphoma through CCL19-Mediated Tumor Cell Retention. Cancer Cell. 36, 250-267.e9 (2019). 31526758

• Description:
  View product citations for Bcl-2 Antibody (OAPB00242) on CiteAb

Target Info

• Gene Symbol: BCL2
• Gene Full Name: BCL2 apoptosis regulator
• Alias Symbols: apoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;PPP1R50;protein phosphatase 1, regulatory subunit 50.
• NCBI Gene Id: 596
• Protein Name: Apoptosis regulator Bcl-2|B-cell CLL/lymphoma 2 [Homo sapiens]
• Description of Target: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
• Uniprot ID: P10415
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAH27258
• Molecular Weight: Predicted: 26kD Observed: 26 kD