BCKDHA Antibody (OAGA03790)

Catalog No: OAGA03790

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA antibody at 1:250 dilution.

BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCKDHA protein stained by BCKDHA antibody [C1C3] diluted at 1:400.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: BCKDHA
• Gene Full Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Alias Symbols: MSU, MSUD1, OVD1A, BCKDE1A
• NCBI Gene Id: 593
• Protein Name: 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
• Description of Target: The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P12694
• Protein Accession #: NP_000700.1
• Nucleotide Accession #: NM_000709.3