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BCKDHA antibody - N-terminal region (ARP56119_P050)

100 ul
$319.00
In Stock

Conjugation Options

ARP56119_P050-FITC Conjugated

ARP56119_P050-HRP Conjugated

ARP56119_P050-Biotin Conjugated

Gene Symbol:
BCKDHA
NCBI Gene Id:
593
Official Gene Full Name:
Branched chain keto acid dehydrogenase E1, alpha polypeptide
Protein Name:
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
Swissprot Id:
P12694
Protein Accession #:
NP_000700
Nucleotide Accession #:
NM_000709
Alias Symbols:
FLJ45695, MSU, MSUD1, OVD1A, BCKDE1A
Replacement Item:
This antibody may replace item sc-105117 from Santa Cruz Biotechnology.
Description of Target:
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-675 BI910860.1 12-686 676-1196 BG742673.1 80-600 1197-1731 BM702667.1 48-582 1732-1763 BE223026.1 1-32 c 1764-1781 BQ018849.1 1-18 c
Protein Size (# AA):
445
Molecular Weight:
50kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
IHC, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express BCKDHA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express BCKDHA.
Immunogen:
The immunogen is a synthetic peptide directed towards the N terminal region of human BCKDHA
Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93%
Complete computational species homology data:
Anti-BCKDHA (ARP56119_P050)
Peptide Sequence:
Synthetic peptide located within the following region: NVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILY
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
UBC; CUL3; BCKDHB; BCKDK;
Blocking Peptide:
For anti-BCKDHA (ARP56119_P050) antibody is Catalog # AAP56119 (Previous Catalog # AAPP37740)
Datasheets/Manuals:
Printable datasheet for anti-BCKDHA (ARP56119_P050) antibody
Sample Type Confirmation:

BCKDHA is supported by BioGPS gene expression data to be expressed in MCF7

Subunit:
alpha, mitochondrial
Target Reference:
Flaschker,N., (2007) J. Inherit. Metab. Dis. 30 (6), 903-909

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